Three people have been diagnosed with mosquito‑borne chikungunya fever in Anzio, 50km south of Rome.
The viral disease causes acute fever and joint pain, and there is no cure, but it is rarely fatal.
It is widespread in the tropics, but the three people in Anzio district are believed to have caught it locally.
Anzio has been ordered to halt blood donations. On 3 September a girl died of cerebral malaria in northern Italy.
Both malaria and chikungunya are very rare in Italy, which drained mosquito‑infested marshes in the 1950s.
The two blood diseases are transmitted by different mosquito species.
It is not clear how the virus arrived in Anzio, but the suspect area has now been fumigated.
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